HEPES Buffer (1M), 100mL

A sterile, ready-to-use 1M HEPES buffer solution supplied in a 100 mL bottle for life science research. HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic biological buffer with a pKa of approximately 7.55 at 25°C, making it especially effective for maintaining physiological pH in the 6.8 to 8.2 range. This pre-made 1M stock eliminates the need to weigh and pH-adjust raw powder, saving bench time and reducing variability between experiments.

Researchers typically dilute it into working solutions for cell culture media, protein purification chromatography, electrophoresis running buffers, and tissue dissociation workflows. The reagent is intended for research use only and should be stored at room temperature, protected from light. STEMCELL Technologies formulates this buffer to complement its cell culture media lines, supporting consistent pH stability during incubations where bicarbonate buffering is insufficient, such as extended manipulations outside a CO2 incubator.