Fragmentation Buffer, 10x

A 10× concentrate reaction buffer engineered by QIAGEN for enzymatic DNA fragmentation, end-repair, and dA-tailing during NGS library preparation. Each vial is calibrated for use at 5 µL per 40 µL reaction master mix, supplying the ionic strength and pH buffering required for reproducible cleavage of purified genomic DNA, amplicons, or cDNA inputs typically ranging from 1 ng to 1 µg. The buffer is qualified alongside the matching QIAGEN fragmentation enzyme chemistry, supporting tunable fragment size selection suitable for Illumina short-read workflows as well as third-party adapters and polymerases. Recommended handling is straightforward: dilute the 10× stock 1:10 in nuclease-free water on ice, mix gently by pipetting (do not vortex), keep reactions chilled during setup, and proceed immediately to the enzyme-addition step. The single-use vial format reduces contamination risk between batches and is shipped ready to integrate with the QIAseq FX DNA Library family of kits, including 24-reaction core configurations. Store at room temperature in the original sealed vial, protect from light, and avoid repeated freeze-thaw cycles to preserve enzymatic activity and buffer integrity across lots. Research use only.