Dulbecco's PBS + 2% FBS

A ready-to-use wash buffer formulated for cell-based assays, Dulbecco's PBS + 2% FBS from STEMCELL Technologies is a phosphate-buffered saline solution supplemented with 2% fetal bovine serum for routine cell washing and as a staining and sorting buffer in flow cytometry workflows. The FBS component provides a protein source that minimizes non-specific antibody binding during immunophenotyping, while the balanced salt formulation supports short-term cell viability during sample preparation, acquisition, and analysis.

This buffer is supplied in a single bottle and is widely adopted for FACS applications, including surface marker staining and cell sorting procedures. Researchers commonly use it to wash cells between antibody incubation steps, resuspend cell pellets, and maintain cell suspensions at refrigerated temperature during multi-color panel staining. The reagent integrates into standard cell culture protocols where gentle handling of live cells is required, and it works with both human and mouse primary cell samples.

Each bottle is intended for in vitro research use; once opened, the solution should be handled under aseptic conditions and refrigerated to preserve the protein supplement. It supports reproducible results across flow cytometry experiments, helping reduce background staining and improve signal-to-noise ratios during data acquisition on common flow cytometry platforms.