Dengue Virus (DENV-NS1 Serotype 4) Ag ≥95%

Recombinant NS1 antigens are essential tools for dengue virus research and diagnostic assay development. This serotype 4 (DENV-4) full length NS1 antigen from Antibodies.com is affinity purified to ≥95% purity and supplied as a 100µg preparation intended for ELISA plate coating and immunization studies.

The NS1 glycoprotein is a non-structural dengue antigen secreted by infected cells and is the principal target for early-phase diagnostic capture assays because it circulates at detectable concentrations during acute infection. A serotype-specific recombinant NS1 reagent allows laboratories to differentiate DENV-4 responses from DENV-1, DENV-2, and DENV-3 in surveillance studies, vaccine immunogenicity testing, and flavivirus co-infection workups.

Typical laboratory uses include coating microplates for indirect IgG or IgM detection assays, serving as a positive control or calibration standard in NS1 antigen capture formats, and immunizing host animals to raise polyclonal or monoclonal antibodies against the DENV-4 NS1 protein. Because the construct covers the complete NS1 coding region, conformational epitopes relevant to clinically characterized antibodies are retained.

For handling, store the reconstituted material frozen at -20°C to -80°C in single-use aliquots to limit freeze-thaw cycles, and protect from light. Work involving dengue virus-derived sequences should follow standard biosafety level 2 precautions appropriate for recombinant flavivirus antigens.