Creatine Kinase MM (CK-MM) Antigen ≥99%

Purified to ≥99% by SDS-PAGE and formulated in Tris-HCl buffer with EDTA and the sulfhydryl-protective reducing agent N-acetyl cysteine, this Creatine Kinase MM antigen from Antibodies.com is supplied as a lyophilized powder in a 100U vial. The muscle-type homodimer isoform of creatine kinase is expressed predominantly in skeletal muscle and is widely used as a biomarker for muscle injury, rhabdomyolysis, and acquired or inherited myopathies. The inclusion of N-acetyl cysteine preserves the catalytically active cysteine residues at the enzyme active site, helping to maintain native conformation through storage and handling.

This preparation is intended for use as an immunogen or calibration standard in immunoassay development, ELISA plate coating, and Western Blot detection workflows. Researchers investigating dystrophinopathies, inflammatory myopathies, and exercise-induced sarcolemmal disruption quantify CK-MM levels as a sensitive indicator of myofiber injury. Reconstitute the lyophilized material in an appropriate aqueous buffer before use, then store aliquots frozen at -20°C for long-term stability; repeated freeze-thaw cycles should be avoided to protect enzymatic activity and the conformational integrity of the antigen.