Buffer RW1 (220 ml)

A proprietary wash buffer from QIAGEN, Buffer RW1 is used as a stringent washing step during RNA purification. According to the manufacturer, it is a proprietary component of RNeasy Kits, intended for washing membrane-bound RNA in RNeasy and AllPrep procedures. The buffer contains a guanidine salt and ethanol, and efficiently removes carbohydrates, proteins, fatty acids and similar biomolecules that are non-specifically bound to the silica membrane, while RNA molecules larger than 200 bases remain bound to the column.

The product is designed to be used with QIAGEN spin-column and automated extraction workflows to support consistent purity in downstream PCR and sequencing applications. The manufacturer notes that Buffer RW1 should not be used for the isolation of small RNAs, such as microRNAs or fragmented RNA from formalin-fixed tissues, as these smaller fragments will be washed away; Buffer RWT should be used instead.

Use as directed in the associated kit protocol and follow appropriate waste handling procedures.