AquaBlock/EIA/WB

This ready-to-use blocking buffer from Antibodies.com is formulated for both ELISA microplate assays and Western blot membrane workflows. The 500 ml liquid volume is supplied in a single-bottle format that removes the weighing, mixing, and filtration steps required when preparing traditional non-fat milk, BSA, or casein blockers. By combining a protein-free polymer matrix with stabilizing surfactants, the reagent minimizes nonspecific antibody binding on polystyrene plate wells and PVDF or nitrocellulose membranes, translating directly into lower background signal and improved signal-to-noise ratios in chromogenic, chemiluminescent, and fluorescent detection readouts. Researchers apply the solution as a one-step blocking step after coating or transfer, and it remains compatible with subsequent antibody incubations without the need for additional washing or detergent supplementation. The formulation also helps suppress cross-reactivity caused by endogenous biotin, rheumatoid factor, or heterophile antibodies in serum-containing samples, supporting more accurate quantification in immunoassay development. Compatible with standard immunodetection workflows, the liquid form simplifies routine bench handling and supports reproducible results across ELISA and WB applications.