Adenovirus Grade 2 Antigen

A partially purified viral preparation from Antibodies.com, this Adenovirus Grade 2 Antigen is concentrated by tangential-flow ultrafiltration and supplied as a 1 mL liquid aliquot for enzyme immunoassay (EIA) workflows. The material serves as a capture or coating reagent in assays that detect adenovirus-specific antibodies or antigen in diagnostic and research samples.

Production begins with propagation of the prototype Grade 2 adenovirus strain in suitable host cells, after which the viral harvest is clarified, inactivated, and processed through ultrafiltration membranes to enrich for viral capsid proteins and structural antigens while reducing background cellular debris. The resulting material retains the immunoreactive epitopes that distinguish serotype 2 from other adenoviral subgroups, supporting its use as a typing reagent in serology panels.

For EIA, the antigen is typically diluted in coating buffer and adsorbed onto microplate wells, where it binds anti-adenovirus antibodies from patient or animal sera. Bound immunoglobulin is then revealed with a species-matched enzyme-conjugated secondary antibody and an appropriate chromogenic substrate. The concentrated format supplied by Antibodies.com allows laboratories to prepare large numbers of coated plates from a single 1 mL vial, reducing inter-batch variability and extending working reagent life. Aliquoting the 1 mL vial into smaller portions before first use is recommended for laboratories that only need small working volumes per experiment, helping preserve antigen activity across multiple freeze-thaw cycles.