PolyFect Transfection Reagent (100ml)

A polycation-based DNA transfection reagent from QIAGEN, optimized for high-efficiency transient transfection of a wide range of mammalian cell lines. The reagent delivers high transfection efficiency with low cytotoxicity in both adherent and suspension cells, and is serum- and antibiotic-compatible for simplified protocols. Its easy-to-use workflow involves mixing DNA with the reagent to form complexes, which are then added directly to cells, and the reagent is suitable for plasmid DNA and co-transfection applications. For storage, it should be kept at 4°C, with repeated freeze–thaw cycles avoided. QIAGEN recommends it for routine gene expression studies, reporter assays, and functional genomics applications that require robust DNA delivery.