Attractene Transfection Reagent (4x1 ml)

QIAGEN's Attractene Transfection Reagent is a lipid-based reagent designed for efficient DNA transfection of eukaryotic cells. It supports a range of applications including plasmid transfection, shRNA vector transfection, protein overexpression, reporter studies, and RNAi experiments. The reagent is compatible with transient transfection, stable transfection, and plasmid co-transfection workflows.

The reagent is reported to work with all cell types, including sensitive and difficult-to-transfect cells. Specific difficult-to-transfect lines mentioned in the listing include HaCaT, MonoMac6, and HCT116, along with suspension cell types such as Jurkat and K562. It is also described as highly suitable for cotransfection of DNA with siRNA or miRNA mimics or inhibitors. Listed features include highly efficient transfection with low toxicity, a rapid fast-forward transfection protocol, freedom from animal-derived components, and the ability to transfect in the presence of serum.

Described as a cationic lipid-based transfection reagent, the product falls within QIAGEN's Assay Technologies portfolio group. Performance figures cited in the supplier listing indicate support for up to 660 transfections in 24-well plates per 1 ml of reagent, with the larger configuration supporting up to 2640 transfections in 24-well plates.