Cotinine-3-HRP Antigen

Designed for sensitive immunoassay workflows, this HRP-labeled Cotinine conjugate is supplied by Antibodies.com as a ready-to-use liquid reagent intended for ELISA-based detection of cotinine, the principal nicotine metabolite widely used as a biomarker of tobacco exposure. The antigen is provided as a 1 mL aliquot in a buffered carrier formulation of 100 mM Tris base at pH 7.0, supplemented with 100 mM fetal bovine serum (FBS), 0.0016 % gentamicin sulfate and 0.005 % thimerosal to preserve enzymatic activity and shelf stability. The conjugate is covalently coupled to horseradish peroxidase (HRP), delivering the strong catalytic turnover required for competitive immunoassays, and is shipped as a liquid antigen–HRP conjugate that integrates directly into standard ELISA plate protocols without further dilution steps. For optimal performance, the reagent should be stored at +4 °C, where it remains stable for up to 12 months; freezing the product is explicitly contraindicated, as ice crystal formation can damage the HRP enzyme and compromise assay reproducibility. Researchers developing competitive ELISAs for nicotine metabolites, building lateral-flow test strips, or screening samples in clinical and forensic toxicology studies will find this conjugate a convenient, well-characterized component for their assay architecture. Each lot is dispatched from the Antibodies.com distribution center in Cambridge, UK, with a typical lead time of 8–12 business days, and the product is sold strictly for research use only and is not intended for diagnostic or therapeutic applications.