Testosterone-3-HRP Antigen

For competitive immunoassay workflows that target small steroid molecules, this ready-to-use Testosterone-3-HRP conjugate from Antibodies.com removes the need for in-house labeling of the hapten. The steroid is covalently linked to horseradish peroxidase through the 3-position, an orientation that generally preserves the immunodominant epitopes on the A and D rings of the steroid backbone, useful when probing antibody specificity raised against the native hormone. Supplied as a liquid conjugate in a 1 ml vial, the reagent can be added directly to coated microplates in a competitive ELISA setup, where labeled antigen and free analyte compete for binding to immobilized antibody. Short-term handling at refrigerated temperatures protected from light preserves enzymatic activity of the reporter, and the solution formulation is intended to avoid repeated freeze-thaw cycling. Researchers developing anti-testosterone monoclonal or polyclonal reagents, or assembling a competitive assay for veterinary, environmental, or clinical research samples, can use this conjugate as the detection component without first optimizing their own conjugation chemistry.