Sheep IgG1 ELISA Kit

Antibodies.com supplies a sandwich ELISA designed for the in vitro quantitative determination of sheep IgG1 in serum, plasma, tissue homogenates, and other biological fluids. The assay is reported to be specific for IgG1 with no observed cross-reactivity with other analogs or variants.

The standard curve covers a concentration range of 1.25–80 ng/mL and the kit's stated sensitivity is <0.75 ng/mL. Detection is colorimetric, read at 450 nm on a standard microplate reader, and the assay is completed in approximately 4 hours on a pre-coated microplate. Sample recovery has been validated in serum (average 95%), EDTA plasma (average 94%) and heparin plasma (average 96%), with dilution-linearity behaviour characterised at 1:2, 1:4 and 1:8 across the same sample types.

The kit is shipped at +4°C and the components are stored at +4°C or −20°C as directed in the protocol manual; freeze/thaw cycles and use past the expiry date should be avoided.