Retinoblastoma (phospho Thr826) Cell Based ELISA Kit

A cell-based ELISA kit from Antibodies.com is designed for the indirect in vitro quantitative determination of Retinoblastoma (phospho Thr826) levels in adherent and suspension cells. The assay uses Anti-Retinoblastoma (phospho Thr826) Antibodies for capture and HRP-conjugated secondary antibodies for colorimetric detection, run on a Tissue Culture Treated Microplate over an assay time of 4 hours 30 minutes. Due to the qualitative nature of this Cell-Based ELISA, multiple normalization methods are described.

The kit is reactive with Human (Thr826), Mouse (Thr819) and Rat (Thr797) samples, with a product range of > 5000 Cells and no reported cross-reactivity. Storage is at +4°C, where it remains stable for 3-6 months. An Anti-GAPDH Antibody is included as an internal positive control in normalizing target absorbance values, Crystal Violet whole-cell staining is used to determine cell density, and an Anti-Retinoblastoma Antibody is provided so the non-phosphorylated target absorbance can be used to normalize the phosphorylated target values.

Synonyms associated with Retinoblastoma include p105-Rb, p110-RB1, pp110, pRb, RB1 and Retinoblastoma-associated protein.