Rat VGF Nerve Growth Factor Inducible ELISA Kit

This sandwich ELISA kit from Antibodies.com is intended for the in vitro quantitative determination of rat VGF in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluids. It employs the sandwich enzyme immunoassay technique using a pre-coated microplate with an antibody specific for VGF. Standards and test samples are added to the wells, after which a Biotinylated Anti-VGF Antibody binds the captured VGF, followed by an HRP-Avidin conjugate and TMB substrate visualisation, with absorbance measured at 450 nm in a microplate reader.

The assay has a stated product range of 31.2–2000 pg/ml and a sensitivity of 12.4 pg/ml. Detection is colorimetric, the platform is a pre-coated microplate arranged as 12 x 8 well strips, and the total assay time is 4h 30m. Kit components are stored at +4°C or −20°C as instructed and must not be used past the expiration date.

Reported average recovery values are 91% for serum, 90% for EDTA plasma, and 84% for heparin plasma, with corresponding ranges of 80–102%, 81–100%, and 80–89% across n=5 samples per matrix. The kit is suitable for serum, plasma, cell lysates, tissue homogenates, and cell culture supernates.