Rat VEGF Co Regulated Chemokine 1 ELISA Kit

Antibodies.com provides a sandwich ELISA kit for the quantitative in vitro determination of rat CXCL17/DMC (VEGF Co-Regulated Chemokine 1) in serum, plasma or other biological fluids. The assay employs a pre-coated 96-well microplate and uses a biotinylated detection antibody together with an HRP-avidin conjugate; signal is developed using TMB substrate and read at 450 nm on a colorimetric microplate reader. Total assay time is 4h 30m.

According to the manufacturer, the kit has a sensitivity of <5.7 pg/ml and an assay range of 15.6-1000 pg/ml. Reported recovery (n=5) is 80%-102% (average 91%) for serum, 81%-100% (average 90%) for EDTA plasma and 80%-89% (average 84%) for heparin plasma. Dilution linearity at 1:2, 1:4, 1:8 and 1:16 is reported as 87-91%, 87-107%, 74-101% and 92-97% for serum; 90-105%, 84-101%, 90-101% and 79-108% for EDTA plasma; and 84-95%, 92-105%, 82-105% and 89-91% for heparin plasma. Components supplied include a pre-coated 96-well microplate, lyophilized standard, Detection Solution A, Detection Solution B, 30X Wash Buffer, Sample Dilution Buffer, TMB Substrate, Stop Solution and plate sealers; kit components should be stored at +4°C or -20°C as instructed and not used past the expiration date.