Rat Urotensin 2 Receptor ELISA Kit

This sandwich ELISA from Antibodies.com is intended for the in vitro quantitative determination of rat GPCR GPR14, also referred to as Urotensin 2 Receptor, in tissue homogenates and other biological fluids. The kit is reactive with rat samples and was validated using serum, EDTA plasma, and heparin plasma, for which both recovery and dilution linearity data are reported.

The assay is performed on a pre-coated 96-well microplate (12 x 8 well strips) using a biotinylated anti-GPCR GPR14 detection antibody and an HRP-avidin conjugate, with the enzymatic reaction visualised using a TMB substrate and quantified by absorbance at 450 nm on a colorimetric microplate reader. According to the supplied specifications, the working range is 0.312-20 ng/ml, the sensitivity is <0.108 ng/ml, and the total assay time is 4h 30m. Reported average recoveries across matrices were 91% (serum), 96% (EDTA plasma) and 94% (heparin plasma), with linearity demonstrated at sample dilutions of 1:2, 1:4, 1:8 and 1:16. Kit components should be stored at +4°C or -20°C as indicated on the individual items and not used past the stated expiration date.