Rat Tryptophan Hydroxylase 2 ELISA Kit

Manufactured for the quantitative in vitro measurement of rat tryptophan hydroxylase 2 (TPH2), this sandwich ELISA from Antibodies.com is intended for use with tissue homogenates, cell lysates and other biological fluids. The assay uses a pre-coated microplate and follows a sandwich enzyme immunoassay workflow employing a Biotinylated Anti-TPH2 Antibody, an HRP-Avidin conjugate and a TMB substrate, with the colour change read at 450 nm on a colorimetric microplate reader. Total assay time is 4 h 30 min.

The stated sensitivity is 0.072 ng/ml and the assay range is 0.156–10 ng/ml. Reported average recoveries across spiked samples are 91% for serum, 90% for EDTA plasma and 84% for heparin plasma, with dilution linearity tested from 1:2 to 1:16 in the same three matrices. Kit components are stored at +4°C or −20°C as instructed, and the kit is not to be used past its expiration date.