Rat Tropomyosin 2 Beta ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com is designed for the in vitro quantitative determination of rat Tropomyosin 2 in tissue homogenates or other biological fluids, with reactivity for rat samples. The kit employs a pre-coated 96-well microplate (12 x 8 well strips) with an antibody specific for Tropomyosin 2, a biotinylated anti-Tropomyosin 2 detection antibody, an HRP-Avidin conjugate, and a TMB substrate that is read at 450 nm.

The stated assay range is 0.312-20 ng/ml and the sensitivity is 0.119 ng/ml, with an assay time of 4h 30m. Reported spike-recovery values include serum 80%-102% (average 91%, n=5), EDTA plasma 81%-100% (average 90%, n=5), and heparin plasma 80%-89% (average 84%, n=5). Dilution linearity data at 1:2, 1:4, 1:8 and 1:16 is provided for serum (74-107%), EDTA plasma (79-108%), and heparin plasma (82-105%).

Kit components include a pre-coated microplate, lyophilized standard, detection solution A (120 µl), detection solution B (120 µl), 30X wash buffer (20 ml), sample dilution buffer (45 ml), TMB substrate (9 ml), stop solution (6 ml), and plate sealers. Components are stored at -20°C or +4°C as specified, and the kit should not be used past the stated expiration date.