Rat Tissue Inhibitors Of Metalloproteinase 4 ELISA Kit

A sandwich enzyme-linked immunosorbent assay (ELISA) from Antibodies.com enables the in vitro quantitative determination of rat TIMP4 in serum, plasma, tissue homogenates, cell lysates, and other biological fluids. The procedure uses a pre-coated 96-well microplate in a 12 x 8 well strip format to capture TIMP4 via an immobilised specific antibody, followed by detection with a biotinylated anti-TIMP4 antibody and an HRP-avidin conjugate. TMB substrate produces a blue product that turns yellow upon addition of acidic stop solution, with absorbance read at 450 nm in a colorimetric microplate reader; the intensity of the yellow colour is proportional to the amount of TIMP4 captured, allowing quantification against a standard curve.

Reported performance figures include a sensitivity of 24 pg/ml, an assay range of 78.1–5000 pg/ml, and a total assay time of 4 hours 30 minutes. Recovery has been evaluated in serum (range 80%–102%, average 91%, n=5), EDTA plasma (range 81%–100%, average 90%, n=5), and heparin plasma (range 80%–89%, average 84%, n=5). Dilution linearity has been tested at 1:2, 1:4, 1:8, and 1:16 dilutions across serum, EDTA plasma, and heparin plasma. Kit components include the pre-coated microplate, lyophilised standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers; components should be stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.