Rat Thrombospondin 2 ELISA Kit

This sandwich ELISA kit from Antibodies.com is designed for the in vitro quantitative determination of rat Thrombospondin 2 in serum, plasma, or other biological fluids. The assay uses a pre-coated 96-well microplate (12 x 8 well strips) and follows a standard sandwich enzyme immunoassay workflow: standards and samples are added to wells coated with an antibody specific for Thrombospondin 2, followed by incubation with a Biotinylated Anti-Thrombospondin 2 Antibody and an HRP-Avidin conjugate. A TMB substrate is then added and the reaction is stopped with acidic stop solution; absorbance is read at 450 nm on a colorimetric microplate reader and the concentration is calculated against a standard curve.

Manufacturer-stated performance characteristics include an assay range of 31.2-2000 pg/ml, a sensitivity of <14.4 pg/ml, and a total assay time of 4 hours 30 minutes. Validation data report recovery of added analyte in serum (80-102%, average 91%), EDTA plasma (81-100%, average 90%), and heparin plasma (80-89%, average 84%), with linearity demonstrated across serial dilutions (1:2 to 1:16) for all three matrices (n=5). Kit components should be stored at +4°C or -20°C as instructed and the product must not be used past its expiration date.