Rat Syndecan 4 ELISA Kit

From Antibodies.com, this sandwich ELISA kit enables the in vitro quantitative determination of rat Syndecan 4 in serum, plasma, tissue homogenates, cell lysates, and other biological fluids. The assay uses a pre-coated 96-well microplate (12 x 8 well strips) and a biotinylated anti-Syndecan 4 detection antibody paired with an HRP-avidin conjugate. Signal is generated by TMB substrate and read as absorbance at 450 nm on a colorimetric microplate reader.

The standard curve covers 0.312–20 ng/ml with a sensitivity of <0.133 ng/ml, and the full protocol runs in approximately 4 hours 30 minutes. Reported mean recovery is 91% for serum, 90% for EDTA plasma and 84% for heparin plasma, with linearity across 1:2 to 1:16 dilutions ranging from 74% to 108% across the same sample types. Components are stored at +4°C or −20°C as instructed, and the kit is intended for research use only.