Rat Sodium/Hydrogen Exchanger 3 ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) is designed for the in vitro quantitative determination of rat Sodium/Hydrogen Exchanger 3/NHE-3 in tissue homogenates, cell lysates or other biological fluids. The assay employs a pre-coated 96-well microplate (12 x 8 well strips) with an antibody specific for the target. Following incubation with standards and samples, a Biotinylated Anti-Sodium/Hydrogen Exchanger 3/NHE-3 Antibody binds the captured analyte, and an HRP-Avidin conjugate is then added. TMB substrate is used to visualise the enzymatic reaction, with absorbance read at 450 nm on a colorimetric microplate reader; the concentration of Sodium/Hydrogen Exchanger 3/NHE-3 is calculated by reference to the standard curve.

The standard curve covers a product range of 0.312–20 ng/ml with a sensitivity of 0.115 ng/ml, and the full assay is completed in 4h 30m. Reported spike-and-recovery data (n=5) include serum (range 80–102%, average 91%), EDTA plasma (range 81–100%, average 90%), and heparin plasma (range 80–89%, average 84%), with dilution linearity reported across 1:2 to 1:16 dilutions for each sample type. Kit components should be stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.