Rat Signal Transducer And Activator Of Transcription 6 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of rat STAT6 (Signal Transducer and Activator of Transcription 6) in serum, plasma, tissue homogenates or other biological fluids. The assay uses a 96-well microplate pre-coated with an antibody specific for STAT6, followed by incubation with a Biotinylated Anti-STAT6 Antibody and an HRP-Avidin conjugate, with signal developed using a TMB substrate solution and read at 450 nm.

Stated sensitivity is <5.8 pg/ml and the assay range is 15.6–1000 pg/ml, with a total assay time of 4h 30m. The kit is supplied with a pre-coated 96-well microplate (12 × 8 well strips), lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers, stored at +4°C or -20°C as instructed. Reported average recovery values are 91% for serum, 90% for EDTA plasma and 84% for heparin plasma.