Rat Signal Transducer And Activator Of Transcription 3 ELISA Kit

This sandwich ELISA from Antibodies.com is designed for the in vitro quantitative determination of rat STAT3 in tissue homogenates, cell lysates, cell culture supernates, and other biological fluids. The assay uses a pre-coated microplate, a biotinylated anti-STAT3 detection antibody, an HRP-avidin conjugate, and TMB substrate. The enzymatic reaction produces a blue product that turns yellow after stop solution is added; absorbance is read at 450 nm and STAT3 concentration is calculated from the standard curve.

Reported sensitivity is <0.054 ng/ml with a working range of 0.156–10 ng/ml. Average recovery values are 91% for serum (range 80–102%, n=5), 90% for EDTA plasma (range 81–100%, n=5), and 84% for heparin plasma (range 80–89%, n=5). Dilution linearity is documented across 1:2, 1:4, 1:8, and 1:16 dilutions for all three sample types.

Total assay time is 4 hours 30 minutes, with colorimetric detection performed on a microplate reader.