Rat SIAE ELISA Kit

From Antibodies.com, this sandwich ELISA is designed for the in vitro quantitative determination of rat SIAE in serum, plasma, tissue homogenates, and other biological fluids. It uses a pre-coated microplate, a biotinylated detection antibody, and HRP-Streptavidin conjugate with TMB substrate, producing a yellow colour read at 450 nm. Total assay time is 4 hours, and components are shipped at +4°C and stored at +4°C or -20°C as instructed in the protocol manual.

The standard curve covers 31.25–2000 pg/mL, with a sensitivity of 18.75 pg/mL. The antibody pair is specific for SIAE, with no reported cross-reactivity with other analogs or variants. Spike-and-recovery in rat samples averaged 91% (range 85–95%, n=10) for serum, 99% (range 86–104%, n=10) for EDTA plasma, and 97% (range 92–103%, n=10) for heparin plasma.

Dilution linearity was confirmed across 1:2, 1:4, and 1:8 dilutions. For serum (n=10), recoveries were 87–92%, 89–99%, and 85–104%, respectively. EDTA plasma (n=10) gave 83–97%, 82–96%, and 85–101%, while heparin plasma (n=10) gave 83–97%, 84–100%, and 80–94%.