Rat Rho Associated Coiled Coil Containing Protein Kinase 2 ELISA Kit

Manufactured for Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (ELISA) is designed for the in vitro quantitative determination of rat ROCK2 (Rho Associated Coiled Coil Containing Protein Kinase 2) in serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids. The assay uses an antibody specific for ROCK2 pre-coated onto a microplate, with a biotinylated anti-ROCK2 detection antibody and an HRP-avidin conjugate, developed with TMB substrate for colourimetric readout.

Quantitative performance spans a measuring range of 0.312–20 ng/ml with a sensitivity of <0.108 ng/ml, and the full protocol completes in 4 hours 30 minutes. Final absorbance is read at 450 nm in a microplate reader, and the concentration of ROCK2 is calculated by reference to a standard curve. Reagent components are stored at +4°C or -20°C as instructed, and the product is intended for research use only, not for diagnostic or therapeutic applications.