Rat Regulator Of G Protein Signaling 6 ELISA Kit

Antibodies.com supplies a Rat RGS6 ELISA Kit configured as a sandwich enzyme-linked immunosorbent assay for the in vitro quantitative determination of rat Regulator Of G Protein Signaling 6 (RGS6) in tissue homogenates or other biological fluids. The assay uses a 96-well pre-coated microplate bearing an antibody specific for RGS6. Standards and samples are added; captured RGS6 is detected using a Biotinylated Anti-RGS6 Antibody followed by an HRP-Avidin conjugate and TMB substrate, producing a yellow product whose absorbance is read at 450 nm on a colorimetric microplate reader.

Reported sensitivity is <0.118 ng/ml with an assay range of 0.312-20 ng/ml, and the total assay time is 4h 30m. Manufacturer validation data report sample recovery for serum (n=5, range 80-102%, average 91%), EDTA plasma (n=5, range 81-100%, average 90%) and heparin plasma (n=5, range 80-89%, average 84%), together with dilution linearity at 1:2, 1:4, 1:8 and 1:16. Kit components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.