Rat Procollagen III N-Terminal Propeptide ELISA Kit

Supplied by Antibodies.com, this sandwich ELISA kit enables in vitro quantitative measurement of rat Procollagen III N-Terminal Propeptide in serum, plasma or other biological fluids [S1]. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, employing a biotinylated detection antibody with HRP-avidin and TMB substrate, and absorbance is read at 450 nm [S1]. The manufacturer-stated range is 31.2–2000 pg/ml with a sensitivity of 13.7 pg/ml [S1], while a third-party listing reports a detection range of 31.25–2000 pg/ml at the same sensitivity [S2]. Total assay time is approximately 4 hours 30 minutes [S1]. Kit components include lyophilized standard, detection solutions, wash buffer, sample dilution buffer, TMB substrate, stop solution and adhesive plate sealers, with storage at +4°C or −20°C as instructed [S1]. A third-party description lists the reagent form as liquid in PBS with 0.02% sodium azide and 50% glycerol at pH 7.3 [S2]. Reported mean recovery values across five samples each are 91% for serum, 91% for EDTA plasma and 84% for heparin plasma [S1], with dilution linearity data provided for serum, EDTA plasma and heparin plasma at 1:2, 1:4, 1:8 and 1:16 dilutions [S1].