Rat Procollagen I N-Terminal Propeptide ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit is designed for the in vitro quantitative determination of rat Procollagen I N-Terminal Propeptide in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, or other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips and employs the sandwich enzyme immunoassay technique with a biotinylated detection antibody and an HRP-avidin conjugate, visualised using TMB substrate solution.

The standard product range is 0.625–40 ng/ml, with a reported sensitivity of 0.215 ng/ml. Absorbance is read at 450 nm on a colorimetric microplate reader, and the total assay time is 4 hours 30 minutes. Reported validation data include average recovery of 91% for serum (range 80–102%, n=5), 90% for EDTA plasma (range 81–100%, n=5), and 84% for heparin plasma (range 80–89%, n=5), with dilution linearity data provided for each sample type across 1:2 to 1:16 dilutions.

Kit components include a pre-coated 96-well microplate, lyophilised standard, detection solutions A and B, 30X wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers. Components are stored at +4°C or –20°C as instructed, and the kit should not be used past its expiration date.