Rat Procollagen I C-Terminal Propeptide ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay from Antibodies.com is designed for the in vitro quantitative determination of rat Procollagen I C-Terminal Propeptide in serum, plasma, or other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips and a biotinylated detection antibody paired with HRP-Avidin and TMB substrate, with the colourimetric signal read at 450 nm on a microplate reader. The reported assay range is 1.25-80 ng/ml with a sensitivity of 0.49 ng/ml, and the total assay time is 4 hours 30 minutes.

Recovery has been evaluated in serum (average 91%, range 80%-102%), EDTA plasma (average 90%, range 81%-100%), and heparin plasma (average 84%, range 80%-89%), with an additional dilution series from 1:2 to 1:16 reporting recovery values between 74% and 108% across the same sample types. Kit components are stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.