Rat Platelet Factor 4 ELISA Kit

This sandwich ELISA from Antibodies.com enables the in vitro quantitative determination of rat Platelet Factor 4 (PF4) in serum, plasma or other biological fluids. The method uses a pre-coated microplate, a biotinylated anti-PF4 detection antibody, an HRP-avidin conjugate, and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader.

Quantitative performance specifications include a sensitivity of <0.128 ng/ml and an assay range of 0.312-20 ng/ml, with a total assay time of 4h 30m. Reported recovery values are 80-102% (average 91%) for serum (n=5), 81-100% (average 90%) for EDTA plasma (n=5), and 80-89% (average 84%) for heparin plasma (n=5). Kit components are stored at +4°C or -20°C as instructed.