Rat PKCZ ELISA Kit

Antibodies.com offers a sandwich ELISA kit for the quantitative detection of rat protein kinase C zeta (PKC zeta) in serum, plasma, tissue homogenates, and other biological fluids. The assay runs in 4 hours on a pre-coated microplate configured as 12 x 8 well strips, with colorimetric detection read at 450 nm. The kit is specific for PKC zeta, with no reported cross-reactivity with other analogs or variants. Reported synonyms for the target include nPKC-zeta, PKC2, PRKCZ, and protein kinase C zeta type.

In validation testing, spike-and-recovery across 10 replicates averaged 93% in serum (range 86%–100%), 96% in EDTA plasma (range 86%–105%), and 98% in heparin plasma (range 91%–103%). Dilution linearity at 1:2, 1:4, and 1:8 dilutions yielded recoveries of 86%–97%, 82%–101%, and 82%–96% in serum; 85%–98%, 88%–100%, and 82%–98% in EDTA plasma; and 89%–101%, 84%–101%, and 83%–93% in heparin plasma, respectively.