Rat Pigment Epithelium Derived Factor ELISA Kit

Antibodies.com supplies a sandwich ELISA for the quantitative in vitro determination of rat Pigment Epithelium Derived Factor (PEDF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The assay is run on a pre-coated microplate arranged as 12 x 8 well strips and uses a biotinylated anti-PEDF detection antibody followed by an HRP-avidin conjugate and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader. The total assay time is 4h 30m, the stated product range is 1.56–100 ng/ml, and the reported sensitivity is <0.55 ng/ml. Kit components are stored at +4°C or -20°C as instructed.

Validation data provided with the kit report the following recoveries (n=5): serum 80%–102% (average 91%), EDTA plasma 81%–100% (average 90%), and heparin plasma 80%–89% (average 84%). Dilution linearity across 1:2 to 1:16 (n=5) was reported as 87–91%, 87–107%, 74–101% and 92–97% for serum; 90–105%, 84–101%, 90–101% and 79–108% for EDTA plasma; and 84–95%, 92–105%, 82–105% and 89–91% for heparin plasma.