Rat Oxytocin Receptor ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay from Antibodies.com is designed for the in vitro quantitative determination of rat Oxytocin Receptor in tissue homogenates, cell lysates, and other biological fluids. The format uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, with a biotinylated anti-Oxytocin Receptor detection antibody and an HRP-Avidin conjugate. TMB substrate is used to visualise the enzymatic reaction, producing a yellow product whose intensity is read at 450 nm on a colorimetric microplate reader. Kit components should be stored at +4°C or -20°C as instructed and not used past the expiration date.

The manufacturer reports a sensitivity of <0.059 ng/ml and an assay range of 0.156–10 ng/ml, with a total assay time of 4 hours 30 minutes. Validation data include spike-and-recovery ranges for serum (80–102%, average 91%), EDTA plasma (81–100%, average 90%), and heparin plasma (80–89%, average 84%), alongside dilution linearity data at 1:2, 1:4, 1:8 and 1:16.