Rat Nuclear Respiratory Factor 1 ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) is designed for the in vitro quantitative determination of rat Nuclear Respiratory Factor 1 (NRF1) in tissue homogenates, cell lysates, and other biological fluids. The kit employs a pre-coated 96-well microplate (12 x 8 well strips), a biotinylated anti-NRF1 detection antibody, an HRP-avidin conjugate, and a TMB substrate solution. The enzymatic reaction produces a colourmetric signal read at 450 nm, with sample NRF1 concentrations calculated from a standard curve.

Performance characteristics include a product range of 0.156–10 ng/ml and a sensitivity of 0.055 ng/ml, with a total assay time of 4 hours 30 minutes. Kit components are stored at +4°C or -20°C as indicated. The kit has been cited in published research on mitochondrial biogenesis and fusion-fission dynamics in rat brain tissue following lead (Pb) exposure during early development.