Rat Norepinephrine Transporter ELISA Kit

Antibodies.com offers a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) for the in vitro quantitative determination of rat Noradrenaline Transporter. The kit is suitable for tissue homogenates, cell lysates, or other biological fluids. The assay employs the sandwich enzyme immunoassay technique, using an antibody specific for Noradrenaline Transporter pre-coated onto a microplate, with detection via a biotinylated antibody and HRP-avidin conjugate, visualised with TMB substrate and read at 450 nm.

The kit delivers a product range of 0.312–20 ng/ml and a sensitivity of <0.115 ng/ml, with results read on a colorimetric microplate reader. The total assay time is 4 hours 30 minutes. Kit components should be stored at +4°C or -20°C as instructed and not used past the expiration date.

Reported sample recovery (n=5) spans 80%–102% with an average of 91% for serum, 81%–100% (average 90%) for EDTA plasma, and 80%–89% (average 84%) for heparin plasma. Dilution linearity (n=5) is provided across 1:2, 1:4, 1:8, and 1:16 dilutions for each matrix. Kit components include a pre-coated microplate, lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers.