Rat Non Metastatic Cells 1, Protein NM23A Expressed In ELISA Kit

This sandwich enzyme-linked immunosorbent assay (sELISA) from Antibodies.com is intended for the in vitro quantitative determination of rat NM23A in serum, plasma or other biological fluids. An antibody specific for NM23A is pre-coated onto the microplate. Standards and test samples are added to the wells; NM23A present in each sample is captured by the immobilized antibody. After incubation and washing, a biotinylated anti-NM23A antibody is added, followed by HRP-avidin conjugate and TMB substrate. The resulting enzymatic reaction is visualised as a blue product that turns yellow upon addition of acidic stop solution, and absorbance is read at 450 nm on a colorimetric microplate reader. The concentration of NM23A in each well is calculated by reference to the standard curve.

Key specifications reported by the manufacturer include a quantitative sandwich format with colorimetric detection, an assay range of 0.312-20 ng/ml, and a sensitivity of <0.108 ng/ml. The kit is validated for rat reactivity and is suitable for use with serum and plasma samples, including EDTA and heparin plasma. Reported total assay time is 4 hours 30 minutes. Kit components are intended to be stored at +4°C or -20°C as instructed, and the kit should not be used past its expiration date.