Rat Nitric Oxide Synthase 3, Endothelial ELISA Kit

This sandwich ELISA is intended for the in vitro quantitative determination of rat endothelial nitric oxide synthase (eNOS) in serum, plasma, tissue homogenates, cell lysates or other biological fluids. It is supplied by Antibodies.com as a pre-coated 96-well microplate (12 x 8 well strips) using a sandwich enzyme immunoassay format, with colorimetric detection read at 450 nm in a microplate reader. Total assay time is 4 hours 30 minutes.

Sensitivity is <5.7 pg/ml and the assay range is 15.6-1000 pg/ml. Average recovery values reported are 91% for serum (range 80-102%), 90% for EDTA plasma (range 81-100%) and 84% for heparin plasma (range 80-89%), each from n=5 samples. The kit includes a lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution and plate sealers, stored at +4°C or -20°C as instructed.