Rat Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4 ELISA Kit

Manufactured by Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) is designed for the in vitro quantitative determination of rat NADPH Oxidase 4 in tissue homogenates, cell lysates or other biological fluids. The assay employs a pre-coated 96-well microplate (12 x 8 well strips) with an antibody specific for NADPH Oxidase 4. Standards and test samples are added to the wells, and captured target is bound by a Biotinylated Anti-NADPH Oxidase 4 Antibody, followed by incubation with HRP-Avidin conjugate. A TMB substrate is used to visualise the HRP enzymatic reaction, producing a blue-coloured product that turns yellow upon addition of acidic stop solution. The density of yellow is proportional to the amount of NADPH Oxidase 4 captured in each well, and concentration is calculated by reading the O.D. absorbance at 450nm in a colorimetric microplate reader and referring to the standard curve.

The assay range is 0.312-20 ng/ml with a sensitivity of 0.128 ng/ml, and total assay time is 4h 30m. Kit components include a pre-coated 96-well microplate, lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, with storage at +4°C or -20°C as instructed. Recovery has been characterised in serum (average 91%, range 80%-102%), EDTA plasma (average 90%, range 81%-100%) and heparin plasma (average 84%, range 80%-89%).