Rat Neurotrophin 4 ELISA Kit

This sandwich Enzyme-Linked Immunosorbent Assay (sELISA) from Antibodies.com is designed for the in vitro quantitative determination of rat Neurotrophin 4 (NT-4). The kit is reactive with rat samples and is listed under the Assays and Kits product category.

The assay has a stated product range of 31.2-2000 pg/ml and a sensitivity of <15.4 pg/ml. It is read on a colorimetric microplate reader with detection at 450 nm, runs on a pre-coated 96-well microplate arranged as 12 x 8 well strips, and has a total assay time of 4 hours 30 minutes. Validated sample types include serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluids. Kit components are stored at +4°C or -20°C as instructed.

In the procedure, an antibody specific for NT-4 is pre-coated onto the plate. Standards and samples are added, followed by washing and incubation with a biotinylated anti-NT-4 antibody and then an HRP-Avidin conjugate. TMB substrate is added and produces a blue product that turns yellow upon addition of acidic stop solution; the yellow intensity is proportional to NT-4 concentration. Reported average recovery values are 91% for serum (range 80-102%, n=5), 90% for EDTA plasma (range 81-100%, n=5), and 84% for heparin plasma (range 80-89%, n=5).