Rat Mothers Against Decapentaplegic Homolog 7 ELISA Kit

Manufactured by Antibodies.com, this assay kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat MADH7/SMAD7 in tissue homogenates, cell lysates or other biological fluids. The kit employs the sandwich enzyme immunoassay technique: a 96-well microtiter plate is pre-coated with an antibody specific for MADH7/SMAD7, standards and test samples are added to the wells, and bound analyte is detected using a Biotinylated Anti-MADH7/SMAD7 Antibody followed by HRP-Avidin conjugate and TMB substrate solution. The enzymatic reaction produces a blue product that turns yellow upon addition of acidic stop solution, and the concentration of MADH7/SMAD7 is calculated from the O.D. absorbance read at 450 nm in a colorimetric microplate reader, referenced to a standard curve.

The kit is intended for use with rat samples and runs on a pre-coated microplate platform with a total assay time of 4 hours 30 minutes. Reported performance specifications include a sensitivity of <0.055 ng/ml and an assay range of 0.156–10 ng/ml. Kit components include a pre-coated 96-well microplate, lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers, with storage temperatures of +4°C or -20°C as indicated. The product is classified under the Assays and Kits category.