Rat Microtubule Associated Protein Tau ELISA Kit

Manufactured by Antibodies.com, this sandwich enzyme-linked immunosorbent assay (sELISA) is designed for the in vitro quantitative determination of rat Tau in tissue homogenates, cell lysates, cell culture supernates, or other biological fluids. The procedure uses a pre-coated 96-well microplate and relies on a biotinylated anti-Tau detection antibody followed by an HRP-avidin conjugate and TMB substrate, with absorbance read at 450 nm on a colorimetric microplate reader.

The assay has a product range of 78.1–5000 pg/ml and a sensitivity of 25 pg/ml, with a total run time of 4 hours 30 minutes. Reported average recovery is 91% in serum (range 80%–102%), 90% in EDTA plasma (range 81%–100%), and 84% in heparin plasma (range 80%–89%), each based on n = 5 samples.

Provided components include a pre-coated 96-well microplate, lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and plate sealers. Storage temperatures between +4°C and -20°C are recommended as instructed for the individual components.