Rat Matrix Metalloproteinase 3 ELISA Kit

From Antibodies.com, this sandwich Enzyme-Linked Immunosorbent Assay (sELISA) enables the in vitro quantitative determination of rat Matrix Metalloproteinase 3 (MMP3) in serum, plasma, and other biological fluids. The assay uses a 96-well microplate pre-coated with anti-MMP3 capture antibody; standards and samples are added, followed by a biotinylated anti-MMP3 detection antibody and HRP-avidin conjugate. Signal is generated with TMB substrate and quantified by absorbance at 450 nm on a colorimetric microplate reader.

The product documentation lists an assay range of 1.25-80 ng/ml and a sensitivity of <0.48 ng/ml, with a total assay time of 4 hours 30 minutes. Validation data report average recovery values of 91% for serum (n=5, range 80%-102%), 90% for EDTA plasma (n=5, range 81%-100%), and 84% for heparin plasma (n=5, range 80%-89%), with dilution linearity assessed at 1:2 through 1:16. Kit components are stored at +4°C or -20°C as indicated, and the kit should not be used past the stated expiration date.