Rat Matrix Metalloproteinase 12 ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of rat Matrix Metalloproteinase 12 (MMP12) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, or other biological fluids. The assay uses a pre-coated 96-well microplate arranged as 12 x 8 well strips, and results are read on a colorimetric microplate reader at 450 nm.

The reported assay range is 78.1-5000 pg/ml with a sensitivity of <32 pg/ml, and the total assay time is approximately 4 hours 30 minutes. Kit components are stored at +4°C or -20°C as instructed.

Sample recovery and dilution linearity have been evaluated in serum, EDTA plasma, and heparin plasma. Average recoveries were 91% for serum, 96% for EDTA plasma, and 95% for heparin plasma, with linearity assessed across 1:2, 1:4, 1:8, and 1:16 dilutions.