Rat Macrophage Inflammatory Protein 3 Alpha ELISA Kit

A sandwich ELISA kit from Antibodies.com designed for the quantitative determination of rat Macrophage Inflammatory Protein 3 alpha in serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. The assay employs the sandwich enzyme immunoassay technique using a pre-coated microplate and a colorimetric microplate reader measuring absorbance at 450 nm.

The kit has a product range of 6.25-400 pg/ml with a sensitivity of less than 2.45 pg/ml. Total assay time is 4 hours 30 minutes. Standards and samples are incubated with a biotinylated detection antibody, followed by HRP-avidin conjugate and TMB substrate, which produces a yellow color proportional to target concentration.

Components include a pre-coated microplate, lyophilized standard, detection solutions A and B, wash buffer, sample dilution buffer, TMB substrate, stop solution, and adhesive plate sealers. Storage requirements vary between +4°C and -20°C depending on the component. Validation data reports average recovery of 91% in serum (n=5, range 83-100%), 94% in EDTA plasma (n=5, range 91-98%), and 94% in Heparin plasma (n=5, range 90-99%). Dilution linearity tested at 1:2, 1:4, 1:8, and 1:16 showed recovery ranges of 78-104% across serum, 81-109% across EDTA plasma, and 74-105% across Heparin plasma.