Rat Lymphocyte Function Associated Antigen 1 Alpha ELISA Kit

From Antibodies.com, this sandwich ELISA kit is designed for the in vitro quantitative determination of rat CD11a (Lymphocyte Function Associated Antigen 1 Alpha) in tissue homogenates, cell lysates or other biological fluids.

The assay uses a pre-coated 96-well microplate (12 x 8 well strips) format with a biotinylated anti-CD11a detection antibody and an HRP-avidin conjugate, read at 450 nm on a colorimetric microplate reader. The reported assay range is 0.156–10 ng/ml with a sensitivity of <0.063 ng/ml, and the total assay time is 4h 30m. Manufacturer-supplied recovery data cover serum (n=5, average 91%, range 83–100%), EDTA plasma (n=5, average 96%, range 90–103%) and heparin plasma (n=5, average 95%, range 89–102%), with parallel dilution linearity values for serum (83–110%, 79–100%, 90–98%, 82–108%), EDTA plasma (90–98%, 90–105%, 73–109%, 87–91%) and heparin plasma (88–103%, 77–105%, 95–98%, 87–110%).

Kit components include a pre-coated 96-well microplate, lyophilized standard (2 vials), detection solutions A and B (120 µl each), 30X wash buffer (20 ml), sample dilution buffer (45 ml), TMB substrate (9 ml), stop solution (6 ml) and plate sealers (5 adhesive strips), with components stored at +4°C or −20°C as instructed.