Rat Low Density Lipoprotein Receptor Related Protein 8 ELISA Kit

A sandwich Enzyme-Linked Immunosorbent Assay (ELISA) from Antibodies.com is intended for the in vitro quantitative determination of rat ApoER2 in serum, plasma or other biological fluids. The kit targets Low Density Lipoprotein Receptor Related Protein 8, is reactive with rat samples, and is supplied on a pre-coated microplate configured as 12 x 8 well strips.

Reported performance includes a sensitivity of <13.3 pg/ml and a working range of 31.2-2000 pg/ml. Detection is colorimetric, with absorbance read at 450 nm in a microplate reader, and the total assay time is 4h 30m. Kit components are stored at +4°C or -20°C as indicated, and the kit must not be used past its expiration date.

Recovery data (n=5 per matrix) are reported as: serum 80%-102% (average 91%), EDTA plasma 81%-100% (average 90%) and heparin plasma 80%-89% (average 84%). The assay uses a biotinylated anti-ApoER2 detection antibody and an HRP-avidin conjugate, with TMB substrate producing a yellow colour whose intensity is proportional to the ApoER2 concentration.