Rat Kisspeptin 1 ELISA Kit

Designed for the in vitro quantitative measurement of rat Kisspeptin in serum, plasma, and other biological fluids, the Rat Kisspeptin 1 ELISA Kit from Antibodies.com reports a sensitivity of <8.47 pg/ml [S1, S2]. The manufacturer lists an assay range of 31.2-2000 pg/ml [S1], while a third-party listing reports 31.25-2000 pg/ml [S2]. The stated assay time is 4h 30m [S1]. One source describes the method as a sandwich enzyme immunoassay using a Kisspeptin-specific pre-coated antibody, biotinylated anti-Kisspeptin detection antibody, HRP-avidin, and TMB substrate read at 450 nm [S1]; another source describes a competitive inhibition enzyme immunoassay technique using biotin-labelled antigen and HRP-avidin detection [S2].

Reported recovery ranges for undiluted samples are: serum (n=5) 80%-102% (average 91%), EDTA plasma (n=5) 81%-100% (average 90%), and heparin plasma (n=5) 80%-89% (average 84%) [S1]. Dilution linearity data across 1:2 to 1:16 dilutions is provided for all three sample types [S1]. Kit components include a pre-coated 96-well microplate (12 x 8 well strips), two vials of lyophilized standard, detection solutions A and B (120 µl each), 30X wash buffer (20 ml), sample dilution buffer (45 ml), TMB substrate (9 ml), stop solution (6 ml), and five adhesive plate sealers [S1]. The manufacturer specifies component-specific storage: the microplate, lyophilized standard, detection solutions, and sample dilution buffer are stored at -20°C, while the wash buffer, TMB substrate, and stop solution are kept at +4°C [S1]. The third-party listing recommends storage at 4°C and avoidance of freeze/thaw cycles [S2].