Rat Kallikrein 4 ELISA Kit

From Antibodies.com, this Rat Kallikrein 4 ELISA Kit is a sandwich enzyme-linked immunosorbent assay (sELISA) designed for the in vitro quantitative determination of rat Kallikrein 4 in serum, plasma or other biological fluids. The assay employs an antibody specific for Kallikrein 4 pre-coated onto a microtiter plate, with detection via a biotinylated secondary antibody, HRP-avidin conjugate, and TMB substrate solution. The reaction is read at 450 nm using a colorimetric microplate reader.

Sensitivity is reported as <0.052 ng/ml, with an assay range of 0.156-10 ng/ml. Total assay time is 4h 30m. Reported recovery in serum (n=5) is 80%-102% with an average of 91%, in EDTA plasma (n=5) is 81%-100% with an average of 90%, and in heparin plasma (n=5) is 80%-89% with an average of 84%.

Contents include a pre-coated microplate, lyophilized standard, detection solutions A and B, wash buffer (30X), sample dilution buffer, TMB substrate, stop solution, and plate sealers. Storage conditions range between +4°C and -20°C depending on component.